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Many customers run their analyzers using reagents that have recently expired, and claim they don’t see a difference in their results. While an analyzer may seem to operate normally when using outdated reagents there is no assurance the results are accurate. Best practices state reagent use should be restricted to the dates defined by the manufacturer. Usage beyond the expiration date puts a patient at risk because the generation of erroneous data may lead to an incorrect and potentially harmful diagnosis. Extended use of expired reagents may also cause damage to the instrument.

Yes, according to ASVCP guidelines, all hematology instruments should have controls run routinely, even systems with internal calibrators. A commitment to consistently repeatable and accurate, quality results involves regular monitoring using commercially available control materials with known target values. Quality control (QC) is needed to verify instrument performance, reagent performance, and operator performance. It is strongly recommended that controls be run daily and be documented to note trends and predict out of control situations before they present result inaccuracies.

Liquid or “wet” chemistry analyzers are the most traditional chemistry system and are commonly used in high-volume reference labs. Sample is added to a liquid reagent which after a reaction over time produces a colored mixture. Light of a particular wavelength is passed through the solution, measuring the reflectance or absorbance of the color by a photodector. The degree of color change is directly or indirectly proportional to the concentration of the analyte or enzyme activity. Dry chemistry systems use reagent strips or slides, impregnated with chemistry reagents in a dried form. When a sample is applied to the reagent strip or slide, it triggers a reaction that results in color development. A photometer is then used to measure the amount of light absorbed or reflected from the surface. Both technologies will provide accurate results when a sample is collected and processed correctly and the analyzer is operated and maintained properly.